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Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and <t>BD-10</t> model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.
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Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and <t>BD-10</t> model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.
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Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and <t>BD-10</t> model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.
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Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and <t>BD-10</t> model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.
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Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and <t>BD-10</t> model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.
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Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and <t>BD-10</t> model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.
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Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and <t>BD-10</t> model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.
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Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and <t>BD-10</t> model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.
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Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and <t>BD-10</t> model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.
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Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and <t>BD-10</t> model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.
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Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and <t>BD-10</t> model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.
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Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and <t>BD-10</t> model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.
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Image Search Results


Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and BD-10 model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.

Journal: bioRxiv

Article Title: NeuronMotif: Deciphering transcriptional cis-regulatory codes from deep neural networks

doi: 10.1101/2021.02.10.430606

Figure Lengend Snippet: Motif discovery performance for different layers in different models. a , Accuracy analysis for discovered motifs of different models. Three columns of box plots describe similarity between neuron motifs and JASPAR motifs in Basset, BD-5 and BD-10 model respectively. For each selected layer in the model, -log10(q-values) distribution of the top 100 JASPAR motifs matched neuron (q-value < 0.1) are shown with box and jittering points. The color of the box means the applied interpretation method. In the first row, it shows the result of input (first) layer of Basset model and shallow layers in BD-5 and BD-10 model with similar receptive field sizes (18 bp) of the Basset input layer neuron (19 bp). In the second row, it shows the convolutional output layer, the convolutional layer in the front of dense layer, result of the three models. b , The number of motifs discovered (q-value < 0.001) from the neuron in convolutional output layer of Basset, BD-5 and BD-10 model. c , The number of motif discovered (q-value < 0.01) from the neuron in layer 3 of Basset model using different interpretation methods including Kelley et al., Alipanahi et al. and NeuronMotif. d , Discovered motifs from the neuron of top convolutional layer in BD-10 model (q-value < 0.01). These motifs can be matched to JASPAR database. Only the one with smallest q-value for each JASPAR motif is shown.

Article Snippet: For example, we built a motif dictionary from layer 10 (L10) in BD-10 model.

Techniques:

NeuronMotif diagnose model defects and guide DCNN architecture design for better performance. a , Dead kernel definition. Dead neuron (pink) activation value distribution is negative. It will be filtered by ReLU activation function. The output of dead neuron is zero. The downstream neuron output does not depend on this neuron. b , c , Diagnosis of motif mixture in the decoupled motifs from Basset, BD-10, DeepSEA and DD10 model. Each point is a decoupled motif generate by a sample set of sequence. The points of motifs generated by the sample set with less than 100 sequences are marked by red color. Otherwise, they are marked by blue color. The distribution of the max activation value is used to show if the relative max activation values of most of the motifs are too low. b , Diagnosis of models trained by DeepSEA dataset c , Diagnosis of models trained by Basset dataset. Only the max activation value of the decoupled motifs in are significantly higher than the decoupled motifs of other neurons in layer 3 of Basset-3 model. d , The meaning of each region in the sub-plots of b , c . e , Schematic for receptive field coupling of previous layer neuron in the neuron sub-structure. f,g , Use AUPRC as an indicator to compare the prediction performance of models. For each model pair, one-sided t-test of Δ AUPRC = AUPRC y-axis – AUPRC x-axis is used to access model performance difference level. f , Comparison between DeepSEA and DD-10 models. g , Comparison between Basset and BD-10 models.

Journal: bioRxiv

Article Title: NeuronMotif: Deciphering transcriptional cis-regulatory codes from deep neural networks

doi: 10.1101/2021.02.10.430606

Figure Lengend Snippet: NeuronMotif diagnose model defects and guide DCNN architecture design for better performance. a , Dead kernel definition. Dead neuron (pink) activation value distribution is negative. It will be filtered by ReLU activation function. The output of dead neuron is zero. The downstream neuron output does not depend on this neuron. b , c , Diagnosis of motif mixture in the decoupled motifs from Basset, BD-10, DeepSEA and DD10 model. Each point is a decoupled motif generate by a sample set of sequence. The points of motifs generated by the sample set with less than 100 sequences are marked by red color. Otherwise, they are marked by blue color. The distribution of the max activation value is used to show if the relative max activation values of most of the motifs are too low. b , Diagnosis of models trained by DeepSEA dataset c , Diagnosis of models trained by Basset dataset. Only the max activation value of the decoupled motifs in are significantly higher than the decoupled motifs of other neurons in layer 3 of Basset-3 model. d , The meaning of each region in the sub-plots of b , c . e , Schematic for receptive field coupling of previous layer neuron in the neuron sub-structure. f,g , Use AUPRC as an indicator to compare the prediction performance of models. For each model pair, one-sided t-test of Δ AUPRC = AUPRC y-axis – AUPRC x-axis is used to access model performance difference level. f , Comparison between DeepSEA and DD-10 models. g , Comparison between Basset and BD-10 models.

Article Snippet: For example, we built a motif dictionary from layer 10 (L10) in BD-10 model.

Techniques: Activation Assay, Sequencing, Generated